About our group :: proteostasisgroup

Proteostasis group
Laboratory of Cell Signalling

Institute of Microbiology

Czech Academy of Sciences

Group members:

Tomáš Groušl - group leader
(ORCID: 0000-0002-6996-1323)
https://www.researchgate.net/profile/Tomas-Grousl

Věra Chvalová - PhD student
(ORCID: 0009-0001-2321-3098)
https://www.researchgate.net/profile/Vera-Chvalova

Vignesh Venkadasubramanian - PhD student
(ORCID: 0000-0003-1700-7439)
https://www.researchgate.net/profile/Vignesh-Venkadasubramanian

Miriam Speváková - Bachelor student

Alumni:

Marina Sokol (2020-2023)

Kateřina Rollerová (2024-2026)

Summer school:

Veronika Lišková (UCT Prague)

Collaborators:

Aleš Vančura - Laboratory of Epigenetics (St. John's College of Liberal Arts and Science)

Axel Mogk - Biogenesis & quality control of proteins (Zentrum für Molekulare Biologie der Universität Heidelberg)

Ayala Shiber - Faculty of Biology (Technion - Israel Institute of Technology)

Christof Taxis - Faculty of Medicine (Health and Medical University Erfurt)

Jana Vojtová - Laboratory of Regulation of Gene Expression (Inst. of Microbiology of the Czech Academy of Sciences)

Mark Rinnerthaler - Genetics and Biology of Aging (Paris Lodron University Salzburg)

Olga Zimmermannová - Laboratory of Membrane Transport (Inst. of Physiology of the Czech Academy of Sciences)

Roman Holič - Institute of Animal Biochemistry and Genetics (Centre of Biosciences of the Slovak Academy of Sciences)

Publications in preparation:

Venkadasubramanian V., Chvalova V., Sokolova M., Rasl J., Grusanovic J., Marvalova O., Vomastek T., Grousl T.:
The sequestration of RACK1 to stress granules is driven by a multiple-component mechanism
Exp Cell Res

Grousl T., Vojtova J., Vomastek T.:
Revealing the assemblies of yeast stress granules and Processing bodies by expansion and super-resolution microscopy
Frontiers in Bioscience-Landmark (resubmission)

Grousl T., Vomastek T.:
The unexpected role of MCC/eisosome plasma-membrane microdomain in the regulation of mRNA decay
TBD

Grousl T., Vojtova J., Vomastek T.:
Ultrastructure expansion microscopy protocol adapted for yeast cells expressing genomically encoded fluorescently labelled proteins
Journal of Microscopy (resubmission)

Publications under review:

Grousl T.:
Yeast P-bodies at a glance
Frontiers in Bioscience-Landmark

Chvalova V., Sokolova M., Vanatko O., Rollerova K., Vomastek T., Grousl T.:
Oxidative stress induces recruitment of MORG1 to PML nuclear bodies
BBRep

Publications of the group members:

Reiter Ch., Ebner P., Kronsteiner E., Moustakakis S., Wiederstein M., Obermayer A., Huber R.,
Schloegerholfer Ch., Wieser F., Richter J., Guirsch I., Rockenfeller P., Vojtova J., Grousl T.,
Felder T., Rinnerthaler M.:
Lipid Droplets as Cellular Garbage Trucks: The Transport of Protein Aggregates to the Vacuole
in Saccharomyces cerevisiae
The FEBS Journal; in press

Vojtova J., Capek M., Willeit S., Grousl T., Chvalova V., Valasek L.S., Kutejova E., Pevala V., Rinnerthaler M.:
A fully automated morphological analysis of yeast mitochondria from wide-field fluorescence images
Scientific Reports. 2024 Dec 3;14(1):30144. doi: 10.1038/s41598-024-81241-0.

Zmuda M., Sedlackova E., Pravdova B., Cizkova M., Cerny O., Allsop T., Grousl T., Malcova I., Kamanova J.:
The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
mBio, 2024 Dec 11;15(12):e0192524. doi: 10.1128/mbio.01925-24.

Rasl J., Caslavsky J., Grusanovic J., Chvalova V., Kosla J., Adamec J., Grousl T., Klimova Z., Vomastek T.:
Depletion of calpain2 accelerates epithelial barrier establishment and reduces growth factor-induced cell scattering
Cell Signal. 2024 Sep;121:111295. doi: 10.1016/j.cellsig.2024.111295.

Grousl T., Vomastek T. (2024):
Xrn1 exoribonuclease – an intrinsic marker of yeast population growth
Front Biosci (Elite Ed). 2024 Jan 31;16(1):1. doi: 10.31083/j.fbe1601001.

Chvalova V., Vomastek T., Grousl T. (2024):
Comparison of holotomographic microscopy and coherence-controlled holographic microscopy
Journal of Microscopy, 2024 Apr;294(1):5-13. doi: 10.1111/jmi.13260.
Chvalova V.; Venkadasubramanian V.; Klimova Z.; Vojtova J.; Benada O.; Vanatko O.; Vomastek T.; Grousl T. (2023):
Characterization of RACK1-depleted mammalian cells by a palette of microscopy approaches reveals defects in cell cycle progression and polarity establishment
Exp Cell Res. 2023 (430) 113695. doi.org/10.1016/j.yexcr.2023.113695
Jawed A., Ho Ch.T., Grousl T., Shrivastava A., Ruppert T., Bukau B., Mogk A. (2023):
Balanced activities of Hsp70 and the ubiquitin proteasome system underlie cellular protein homeostasis
Front Mol Biosci. 2023 Jan 4;9:1106477. doi: 10.3389/fmolb.2022.1106477.

Rasl J., Grusanovic J., Klímová Z., Caslavsky J., Grousl T., Novotny J., Kolar M., Vomastek T. (2022):
ERK2 signaling regulates cell-cell adhesion of epithelial cells and enhances growth factor-induced cell scattering
Cell Signal. 2022 Nov;99:110431. doi: 10.1016/j.cellsig.2022.110431.
Grousl T.#, Vojtova J., Hasek J., Vomastek T. (2021):
Yeast stress granules at a glance.
Yeast. 2022 Apr;39(4):247-261. doi: 10.1002/yea.3681.
# Corresponding author

Ramaniuk O., Klimova Z., Grousl T.#, Vomastek T.# (2020):
Quantitative Phase Imaging of Spreading Fibroblasts Identifies the Role of Focal Adhesion Kinase in the Stabilization of the Cell Rear.
Biomolecules. 2020. doi: 10.3390/biom10081089.
# Corresponding authors

Ho Ch.T., Grousl T., Shatz O., Jawed A., Ruger-Herreros C., Semmelink M., Zahn R., Richter K., Bukau B., Mogk M. (2019):
Cellular sequestrases maintain basal Hsp70 capacity ensuring balanced proteostasis.
Nat Commun. 2019. doi: 10.1038/s41467-019-12868-1.

Grousl T.*, Ungelenk S.*, Miller S.*, Ho Ch.T., Khokhrina M., Mayer M.P., Bukau B., Mogk A. (2018):
A prion-like domain in Hsp42 drives chaperone-facilitated aggregation of misfolded proteins.
J Cell Biol. 2018 Jan 23 pii: jcb.201708116
* These authors contributed equally to this work.

Ungelenk S., Moayed F., Ho Ch.T., Grousl T., Scharf A., Mashaghi A., Tans S., Mayer M.P., Mogk A. & Bukau B. (2016):
Small heat shock proteins sequester misfolding proteins in near-native conformation for cellular protection and efficient refolding.
Nature Commun., 2016 Nov 30;7:13673. doi: 10.1038/ncomms13673.
Poornima G., Shah S., Vignesh V., Parker R., Rajyaguru P.I. (2016):
Arginine methylation promotes translation repression activity of eIF4G-binding protein, Scd6
Nucleic Acids Res. 2016 Nov 2;44(19):9358-9368. doi: 10.1093/nar/gkw762

Cherkasov V.*, Grousl T.*, Theer P., Vainshtein Y., Glaßer C., Mongis C., Kramer G., Stoecklin G., Knop M., Mogk A.,
Bukau B.(2015):
Systemic control of protein synthesis through coordinated sequestration of translation and ribosome biogenesis factors during severe heat stress.
FEBS Lett. 2015 Nov 30;589(23):3654-64. doi: 10.1016/j.febslet.2015.10.010.
* These authors contributed equally to this work.

Grousl T.*, Opekarova M.*, Stradalova V., Hasek J., Malinsky J. (2015):
Evolutionarily conserved 5'-3' exoribonuclease Xrn1 accumulates at plasma membrane-associated eisosomes in post-diauxic yeast.
PLoS One. 2015 Mar 26;10(3):e0122770. doi: 10.1371/journal.pone.0122770.
* These authors contributed equally to this work.

Rinnerthaler M., Lejskova R., Grousl T., Stradalova V., Heeren G., Richter K., Breitenbach-Koller L., Malinsky J., Hasek J., Breitenbach M. (2013):
Mmi1, the Yeast Homologue of Mammalian TCTP, Associates with Stress Granules in Heat-Shocked Cells and Modulates Proteasome Activity.
PLoS ONE 8 (10). doi:10.1371/journal.pone.0077791.

Grousl T., Ivanov P., Malcova I., Pompach P., Frydlova I., Slaba R., Senohrabkova L., Novakova L., Hasek J. (2013):
Heat Shock-Induced Accumulation of Translation Elongation and Termination Factors Precedes Assembly of Stress Granules in S. Cerevisiae.
PLoS ONE 8 (2). doi:10.1371/journal.pone.0057083.

Rinnerthaler M., Buttner S., Laun P., Heeren G., Felder T.K., Klinger H., Weinberger M., Stolze K., Grousl T., Hasek J., Benada O., Frydlova I., Klocker A., Simon-Nobbe B., Jansko B., Breitenbach-Koller H., Eisenberg T., Gourlay C.W., Madeo F., Burhans W.C., Breitenbach M. (2012):
Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast.
Proc Natl Acad Sci USA. 2012 May 29;109(22):8658-63. doi: 10.1073/pnas.1201629109.

Grousl T., Ivanov P., Frydlova I., Vasicova P., Janda F., Vojtova J., Malinska K., Malcova I., Novakova L., Janoskova D., Valasek L., Hasek J. (2009):
Robust Heat Shock Induces eIF2alpha-Phosphorylation-Independent Assembly of Stress Granules Containing eIF3 and 40S Ribosomal Subunits in Budding Yeast, Saccharomyces Cerevisiae.
Journal of Cell Science 122 (Pt 12): 2078-88. doi:10.1242/jcs.045104.

Prevorovsky M., Grousl T., Stanurova J., Rynes J., Nellen W., Puta F., Folk P. (2009):
Cbf11 and Cbf12, the Fission Yeast CSL Proteins, Play Opposing Roles in Cell Adhesion and Coordination of Cell and Nuclear Division.
Experimental Cell Research 315 (8): 1533-47. doi:10.1016/j.yexcr.2008.12.001.